Yx. Mak et Pm. Doran, EFFECT OF CELL-CYCLE INHIBITION ON SYNTHESIS OF STEROIDAL ALKALOIDS BY SOLANUM-AVICULARE PLANT-CELLS, Biotechnology letters, 15(10), 1993, pp. 1031-1034
Addition of 40 mg l-1 hydroxyurea to Solanum aviculare cultures result
ed in complete inhibition of growth and an increase in the levels of s
teroidal alkaloids in the biomass. These results demonstrate that sola
sodine synthesis and growth can be uncoupled, and suggest that steroid
al-alkaloid production can be significantly enhanced by inhibiting gro
wth at high cell density. Treatment with 1 mg l-1 abscisic acid had a
negligible effect on growth and product synthesis.