DETERMINATION OF FUROSINE IN HAIR BY LIQUID-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY

Citation
A. Takemura et al., DETERMINATION OF FUROSINE IN HAIR BY LIQUID-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY, BMC. Biomedical chromatography, 11(1), 1997, pp. 61-62
Citations number
8
Categorie Soggetti
Chemistry Analytical","Pharmacology & Pharmacy",Biology,"Biochemical Research Methods
ISSN journal
02693879
Volume
11
Issue
1
Year of publication
1997
Pages
61 - 62
Database
ISI
SICI code
0269-3879(1997)11:1<61:DOFIHB>2.0.ZU;2-U
Abstract
A sensitive and reliable analytical method for determining furosine in hair has been developed using liquid chromatography-tandem mass spect rometry, Fructose-N-alpha-formyl-d(4)-DL-lysine was synthesized and us ed as an internal standard, By the present method, glycated lysine lev els in hair could be determined as fructose-lysine in only 0.1 mg of h air sample, ranging from 35.1-72.6 ng/mg hair among five healthy volun teers, which corresponded to 0.0635-0.153% of the total lysine content s in hair determined by amino acid analysis.