V. Sreenivasan et al., BREFELDIN-A AFFECTS WEST NILE VIRUS-REPLICATION IN VERO CELLS BUT NOTC6 36 CELLS/, Journal of virological methods, 45(1), 1993, pp. 1-17
A fungal metabolite brefeldin A (BFA) was used to study virus-host int
eraction in glycoprotein processing in West Nile virus-infected Vero a
nd C6/36 cells. The results indicated that as little as 1 mug/ml of BF
A resulted in complete breakdown in the Golgi organelle in infected Ve
ro cells. This led to modifications of the glycoproteins which could n
ot be efficiently used in infectious virion formation. In contrast, as
much as 10 mug/ml of BFA in culture medium did not affect either glyc
oprotein formation or production of infectious particles in C6/36 cell
s. The results showed that in Vero cells, the transport of glycoprotei
ns to the Golgi apparatus is important in West Nile virus infection. I
t also showed that BFA could be used as a tool to understand further t
he trafficking of glycoprotein from the ER to Golgi in flavivirus infe
ction in Vero cells.