BREFELDIN-A AFFECTS WEST NILE VIRUS-REPLICATION IN VERO CELLS BUT NOTC6 36 CELLS/

Citation
V. Sreenivasan et al., BREFELDIN-A AFFECTS WEST NILE VIRUS-REPLICATION IN VERO CELLS BUT NOTC6 36 CELLS/, Journal of virological methods, 45(1), 1993, pp. 1-17
Citations number
24
Categorie Soggetti
Virology,"Biochemical Research Methods
ISSN journal
01660934
Volume
45
Issue
1
Year of publication
1993
Pages
1 - 17
Database
ISI
SICI code
0166-0934(1993)45:1<1:BAWNVI>2.0.ZU;2-K
Abstract
A fungal metabolite brefeldin A (BFA) was used to study virus-host int eraction in glycoprotein processing in West Nile virus-infected Vero a nd C6/36 cells. The results indicated that as little as 1 mug/ml of BF A resulted in complete breakdown in the Golgi organelle in infected Ve ro cells. This led to modifications of the glycoproteins which could n ot be efficiently used in infectious virion formation. In contrast, as much as 10 mug/ml of BFA in culture medium did not affect either glyc oprotein formation or production of infectious particles in C6/36 cell s. The results showed that in Vero cells, the transport of glycoprotei ns to the Golgi apparatus is important in West Nile virus infection. I t also showed that BFA could be used as a tool to understand further t he trafficking of glycoprotein from the ER to Golgi in flavivirus infe ction in Vero cells.