IMMOBILIZATION OF NUCLEASES ON CORN COB

A modification of the activation methodology of hydroxylic support usi ng p-toluensulfonyl chloride is described. The methodology is applied to the corn cob. The influence of several treatments of the support an d of the presence of pyridine or ethanolamine in the activation proces s is discussed. Endonuclease Staphylococcus aureus and pancreatic ribo nuclease are immobilized by this methodology. The enzymatic derivative s are active in the hydrolysis of RNA and DNA (only endonuclease S. au reus).