THE FREQUENCY OF OCCURRENCE OF ACTINOMYCES-PYOGENES AND ACTINOMYCES LIKE ORGANISMS (ALO) IN ENDOMETRIUM BIOPSY OF COWS FROM VOJVODINA AND BARANJA

The aim of the study was to determine the prevalence of Actinomyces py ogenes and ''ALO'' in the endometrium of cows in this geographical reg ion. It included 102 animals from 12 heards in the period from October 6. 1994. to may 10. 1995. Sampling was performed with Folmer-Nielsen catheters, as modified by Jovic. Samples were seeded on solid media im mediately after callection under field conditions. Aerobic incubation was begun on the same day at 37 degrees C. The primary isolates, their subcultures and colonies obtained after passages in liquid media were examined for colony morphology and staining characteristics, catalase , oxidase, CAMP phenomena and biochemical activity. The biochemical id entity of Actinomyces pyogenes was confirmed using the API Coryne syst em (Bio Merieux, Marny, L'Etoil, France). The ''ALO'' identity was est ablished by excluding parameters guoted as crucial. Every coryneform o rganism which caused beta haemolysis on blood agar but was not designa ted as Actinomyces pyogenes, Arcanobacterium haemolyticum, Corynebacte rium pseudotuberculosis, Corynebacterium ulcerans and beta strains of Erysipelothrix rhusiopathiae was identified as ''ALO''.