Chemical and microbiological properties of plain nonfat yogurt were de
termined after 2, 6 and 12 days refrigerated storage. Sensory properti
es were determined after 6 days storage. Viable culture bacteria conce
ntrations ranged from 140 to 8,000 x 10(6)/g and ratios of lactobacill
us to streptococcus ranged from 0. 18 to 15.4. Chemical criteria used
to characterize products included: pH, titratable acidity, lactic and
four other organic acids and lactose by HPLC, and 23 major volatile or
ganic compounds by dynamic headspace analysis. Lactic acid concentrati
ons ranged from 7.5 to 9.9 mg/g. Major flavor volatiles in all yogurts
included: acetaldehyde, heptane, acetone, diacetyl, and benzothiazole
. Untrained sensory panels showed differences for flavor, aroma and ac
ceptability.