FORMATION OF EPSILON-(GAMMA-GLUTAMYL) LYSINE CROSS-LINK IN CURED HORSE MACKEREL MEAT INDUCED BY DRYING

Block meat cut from horse mackerel was cured in 3M NaCl (pH 7.5) in th e presence and absence of 5 mM EDTA for 3 hr at 4-degrees-C. During dr ying at 30-degrees-C, changes in the myosin heavy chain and epsilon-(g amma-glutamyl)lysine isopeptide bonds in the meat were observed. After 20 hr drying, cross-linking of the myosin heavy chain was observed an d epsilon(gamma-glutamyl)lysine bonds increased eight fold. These chan ges were inhibited in the presence of EDTA. These results suggested th at transglutaminase was probably involved in the cross-linking reactio n of the myosin heavy chain in the manufacture of dried fish (''Himono '' in Japan).