Mp. Boland et al., IDENTIFICATION AND CHARACTERIZATION OF A TYPE-1 PROTEIN PHOSPHATASE FROM THE OKADAIC ACID-PRODUCING MARINE DINOFLAGELLATE PROROCENTRUM-LIMA, FEBS letters, 334(1), 1993, pp. 13-17
The unicellular marine dinoflagellate, Prorocentrwn lima, an establish
ed producer of okadaic acid (OA), was shown to contain a type-1 protei
n phosphatase (PP-1) the biochemical profile of which on Mono-Q and Su
perdex-75 fast protein liquid chromatography was identical to the cata
lytic subunit of PP-1 from rabbit skeletal muscle. Purified P lima PP-
1 (apparent molecular mass 37.5 kDa) was highly sensitive to inhibitio
n by mammalian protein phosphatase inhibitor-1 and inhibitor-2, and to
OA itself. A 6-7-fold increase in OA production by P lima, when grown
under controlled conditions, correlated with an up to 300-fold increa
se in P. lima PP-1 activity. Furthermore, P. lima did not contain any
detectable type-2A protein phosphatase activity. This study represents
the first identification of a serine/threonine protein phosphatase in
a dinoflagellate.