HPLC ASSAY OF MELATONIN IN PLASMA WITH FLUORESCENCE DETECTION

We report an HPLC assay for melatonin that incorporates automated inje ction, methanol/water mobile phase, and fluorescence detection. Plasma samples were extracted by solid and liquid phases. Recovery was > 70% for 1-10 mL of plasma extracted, approximately 40 pg-250 ng of melato nin. Samples were dried and reconstituted in 100 mL/L methanol. Inject ions were 25 muL or 150 muL, depending on sample concentration, and th e melatonin peak was eluted in 380 mL/L methanol. The detection limit of the assay was 6 pg on the column, allowing a practical sensitivity in plasma of 11 pmol/L for 8-mL samples and 34 pmol/L for 2-mL samples . More than 100 plasma samples from volunteers and patients were assay ed and the results compared with an established RIA. The mean daytime concentration of melatonin was 20.7 pmol/L (SEM = 1.2) and 18.5 pmol/L (SEM = 1.6) for HPLC and RIA, respectively, and the mean nighttime co ncentration was 82.4 pmol/L (SEM = 6.5) and 82.2 (SEM = 7.3), respecti vely.