Koa. Chin et al., A CONSTANT COMPOSITION KINETICS STUDY OF THE INFLUENCE OF SALIVARY CYSTATINS, STATHERIN, AMYLASE AND HUMAN SERUM-ALBUMIN ON HYDROXYAPATITE DISSOLUTION, Colloids and surfaces. A, Physicochemical and engineering aspects, 78, 1993, pp. 229-234
The influence of salivary cystatins, statherin, amylase and human seru
m albumin on the dissolution kinetics of hydroxyapatite has been inves
tigated using the constant composition method. At a pH of 6.0, salivar
y cystatins and statherin all decreased the dissolution rate by about
30% at a surface concentration of 5.0 x 10(-8) mol m-2. Although littl
e difference between neutral cystatin SN and acidic cystatin S was see
n at pH 6.0, when the reaction was performed at a similar degree of un
dersaturation at neutral pH (6.95), cystatin S was the more efficient
inhibitor. At a surface concentration of 5.0 x 10(-8) mol m-2, cystati
n SN and cystatin S decreased the rates of dissolution by 35% and 30%
at pH 6.0 and by 40% and 65% at a pH of 6.95 respectively. Human serum
albumin and amylase were more efficient dissolution inhibitors than e
ither of these salivary cystatins at pH 6.0 and 4.8. Moreover, in all
cases the influence of the adsorbed proteins increased with extent of
reaction.