The swelling and the surface charge of birch kraft pulps affected the
hydrolysis of pulp xylan with Trichoderma reesei and Bacillus circulan
s xylanases. The more swollen the fiber, the less it was hydrolyzed. F
urthermore, the greater the negative surface charge, the less the pulp
was hydrolyzed Both the swelling and the surface charge could be adju
sted by suitable choice of counterions of the carboxyl groups in the p
ulp, indicating that the counterions in pulp have a profound effect on
the action of xylanases in the pulp matrix.