EFFECTS OF INHIBITORS OF GLYCOSYLATION ON GLYCOSIDASE SECRETION IN ASPERGILLUS-AWAMORI VAR X100 D27/

Using glucoamylase, alpha-galactosidase, and alpha-glucosidase of the micellar fungus Asp. awamori as examples of secreted glycoproteins, th e relationship between glycosylation and secretion was studied using t unicamycin and 2-deoxy-D-glucose as inhibitors of N- and O-glycosylati on, respectively. Tunicamycin suppresses N-glycosylation and thus decr eases the level of secretion of all four glycosidases. 2-Deoxy-D-gluco se, which predominantly suppresses O-glycosylation, has no effect on t he secretion level.