A NEW INSTRUMENT FOR OVERPRESSURED LAYER CHROMATOGRAPHY

A new instrument has been developed for overpressured layer chromatogr aphy. In the instrument the eluent is fed on to the chromatographic pl ate, from below, by a syringe pump. The velocity of the mobile phase i s very stable and is linearly dependent on the migration distance. Gas is used to apply overpressure to the chromatographic plate. The perfo rmance of the new instrument was tested by separating the components o f different mixtures. The separations were much better than those obta ined in classical chromatographic chambers and were achieved in less t ime. The instrument can be set up extremely quickly and is very easy t o operate.