LANGMUIR FILMS FROM ANTIBODIES BASED ON A MPHIPHILIC POLYELECTROLYTES

A method for formation of Langmuir films from antibodies based on an a mphiphilic polyelectrolyte has been developed. The physicochemical and immunochemical properties of the novel Langmuir films were studied. I t was found that the interaction of HBsAg with Langmuir films is descr ibed by the model with one binding site and provides the immunoenzyme assay sensitivity of similar to 0,2 ng/ml. The interaction of HBsAg wi th the antibodies adsorbed on the polysteren plate surface is describe d by the model with the positive cooperativity and provides the assay to reach sensitivity of 2-3 ng/ml.