IMPROVED EXTRACTION AND REVERSED PHASE-HIGH PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF FLAVONOIDS AND THE IDENTIFICATION OF ROSA CULTIVARS

Citation
C. Vansumere et al., IMPROVED EXTRACTION AND REVERSED PHASE-HIGH PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF FLAVONOIDS AND THE IDENTIFICATION OF ROSA CULTIVARS, Phytochemical analysis, 4(6), 1993, pp. 279-292
Citations number
42
Categorie Soggetti
Biology,"Chemistry Analytical","Plant Sciences
Journal title
ISSN journal
09580344
Volume
4
Issue
6
Year of publication
1993
Pages
279 - 292
Database
ISI
SICI code
0958-0344(1993)4:6<279:IEARPP>2.0.ZU;2-8
Abstract
Optimal extraction methods and Reversed-Phase High Performance Liquid Chromatographic (RP-HPLC) procedures were developed for the isolation and resolution of naturally occurring flavonoids and related phenolic compounds in Rosa petals. The following flavonoids and related phenoli cs were identified or partly identified: cyanidin 3,5-di-O-glucoside; cyanidin 3,5-di-O-glycoside (sugar moieties, glucose and arabinose); p elargonidin 3,5-di-O-glycoside; pelargonidin 3-O-glycoside; quercetin 3-O-glucoside; ellagic acid; quercetin 3-O-arabinoside, quercetin 4'-O -glucoside; kaempferol 4'-O-glucoside; kaempferol 3-O-rutinoside; kaem pferol 3-O-glucoside; kaempferol 3-O-arabinoside; kaempferol 3-O-rhamn oside and kaempferol 3-O-glycoside. Significant qualitative and quanti tative differences between the above flavonoid markers allowed an obje ctive distinction between 44 cultivars examined. The production of 'Fl ower Flavonoid RP-HPLC Fingerprints', which have been converted in the corresponding 'Computer Flower Flavonoid Identity Cards' via a suitab le computer program, has proven to be of great value for Rosa cultivar recognition.