T. Teerlink et al., RAPID-DETERMINATION OF GLUTAMINE IN BIOLOGICAL SAMPLES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Clinica chimica acta, 218(2), 1993, pp. 159-168
A high-performance liquid chromatographic method for the determination
of glutamine in biological samples is presented. Glutamine was deriva
tized with ortho-phthalaldehyde reagent containing 3-mercaptopropionic
acid and separated by reversed phase chromatography on a C18 column c
ontaining 3-mum particles. No interference from other amino acids was
observed. The assay was linear over a range from 1 to 2,000 mumol/l. A
nalytical recovery of plasma samples spiked with glutamine was 98.6 +/
- 3.8%. Within- and between-batch imprecision were 1.5% and 2.2%, resp
ectively. The derivatization step was fully automated. Total analysis
time, including derivatization and chromatography, amounted to 6 min.
The method can be used for the determination of glutamine in plasma, u
rine, cerebrospinal fluid and tissue homogenates.