RAPID-DETERMINATION OF GLUTAMINE IN BIOLOGICAL SAMPLES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Citation
T. Teerlink et al., RAPID-DETERMINATION OF GLUTAMINE IN BIOLOGICAL SAMPLES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Clinica chimica acta, 218(2), 1993, pp. 159-168
Citations number
24
Categorie Soggetti
Chemistry Medicinal
Journal title
ISSN journal
00098981
Volume
218
Issue
2
Year of publication
1993
Pages
159 - 168
Database
ISI
SICI code
0009-8981(1993)218:2<159:ROGIBS>2.0.ZU;2-#
Abstract
A high-performance liquid chromatographic method for the determination of glutamine in biological samples is presented. Glutamine was deriva tized with ortho-phthalaldehyde reagent containing 3-mercaptopropionic acid and separated by reversed phase chromatography on a C18 column c ontaining 3-mum particles. No interference from other amino acids was observed. The assay was linear over a range from 1 to 2,000 mumol/l. A nalytical recovery of plasma samples spiked with glutamine was 98.6 +/ - 3.8%. Within- and between-batch imprecision were 1.5% and 2.2%, resp ectively. The derivatization step was fully automated. Total analysis time, including derivatization and chromatography, amounted to 6 min. The method can be used for the determination of glutamine in plasma, u rine, cerebrospinal fluid and tissue homogenates.