RAYLEIGH LIGHT-SCATTERING SPECTROSCOPY - APPLICATION TO THE DETERMINATION OF PROTEINS USING BROMOPYROGALLOL RED

Authors
MA CQ LI KA TONG SY
Citation
Cq. Ma et al., RAYLEIGH LIGHT-SCATTERING SPECTROSCOPY - APPLICATION TO THE DETERMINATION OF PROTEINS USING BROMOPYROGALLOL RED, Bulletin of the Chemical Society of Japan, 70(1), 1997, pp. 129-133
Citations number
20
Categorie Soggetti
Chemistry
Journal title
Bulletin of the Chemical Society of Japan → ACNP
ISSN journal
00092673
Volume
70
Issue
1
Year of publication
1997
Pages
129 - 133
Database
ISI
SICI code
0009-2673(1997)70:1<129:RLS-AT>2.0.ZU;2-R
Abstract
A method for the quantitation of proteins in aqueous solution involvin g the binding of bromopyrogallol red to proteins under acidic conditio ns has been developed. The binding of the dye to proteins is accompani ed by an enhancement of Rayleigh light scattering at 332 nm; the scatt ering intensity is linear over the range 0.136-6.80 mu g ml(-1). The r eaction is completed immediately after mixing dye and protein solution s, and the scattering signal is stable for at least 3 h. There are ver y few interference with the method, most of which can be minimized by dilution.