QUASI-SPECIES NATURE OF HEPATITIS-C VIRUS AND RESPONSE TO ALPHA-INTERFERON - SIGNIFICANCE AS A PREDICTOR OF DIRECT RESPONSE TO INTERFERON

Background/Aims: We evaluated the significance of the quasispecies nat ure of HCV as a predictor of the response to alpha interferon therapy in patients with chronic hepatitis C. Methods: Natural alpha interfero n was administered in 62 patients for 24 weeks (daily for 2 weeks, the n three times weekly for 22 weeks) and factors were analyzed that coul d affect the response. HCV subtype, HCV RNA concentrations and the num ber of HCV quasispecies were evaluated before treatment. HCV RNA conce ntrations were measured by branched DNA probe assay. The number of HCV quasispecies was measured by fluorescence single-strand conformation polymorphism analysis. Results: The HCV RNA concentration (p<0.0001), HCV subtype (p=0.0076), and the number of HCV quasispecies (p=0.0024) were significantly associated with a complete response. Multivariate a nalyses showed that the number of HCV quasispecies was an independent predictor of the disappearance of HCV RNA during the administration of alpha interferon, but did not predict a relapse after its completion. Pretreatment concentration of HCV RNA was the only factor that was re lated to a long-term disappearance of HCV RNA. Conclusions: The number of HCV quasispecies was significantly related to the response to alph a interferon early in its administration. The pretreatment concentrati on of HCV RNA was mainly related to a relapse following completion of treatment.