DETECTION OF THE (11 22)(Q24 Q12) TRANSLOCATION OF EWINGS-SARCOMA ANDPERIPHERAL NEUROECTODERMAL TUMOR BY REVERSE TRANSCRIPTION-POLYMERASE CHAIN-REACTION
Jr. Downing et al., DETECTION OF THE (11 22)(Q24 Q12) TRANSLOCATION OF EWINGS-SARCOMA ANDPERIPHERAL NEUROECTODERMAL TUMOR BY REVERSE TRANSCRIPTION-POLYMERASE CHAIN-REACTION, The American journal of pathology, 143(5), 1993, pp. 1294-1300
Ewing's sarcoma and the related primitive neuroectodermal tumor (PNET)
share a unique and specific t(11;22)(q24;q12) chromosomal translocati
on. The breakpoints have recently been cloned and shown to involve the
EWS gene on chromosome 22 and the FLI-1 gene on chromosome 11. Transl
ocation results in the fusion of these genes on the der(22) chromosome
, resulting in the production of a novel chimeric EWS/FLI-1 message. U
sing oligonucleotide primers derived from EWS and FLI-1 complementary
DNAs, we were able to amplify a specific fusion transcript from 18 of
18 cases containing t(11;22) and 10 of 14 cases of Ewing's sarcoma/PNE
T that had unsuccessful cytogenetics. No EWS/FLI-1 fusion transcripts
were detected in five cell lines derived from cases of pediatric sarco
mas having a histological diagnosis other than Ewing's sarcoma/PNET. T
he sensitivity and specificity of this PCR analysis demonstrates the u
sefulness of this approach for the primary diagnosis of t(11;22)-conta
ining Ewing's sarcoma/PNET and for the detection of metastatic or resi
dual disease.