PROMOTION OF ROOT EMERGENCE IN-VITRO FROM RHIZOME BUDS OF LAPAGERIA-ROSEA CV NASHCOURT AFTER PROLIFERATION IN THE PRESENCE OF PACLOBUTRAZOL

Medium components that may influence the emergence of adventitious roo t primordia in rhizome buds of Lapageria rosea (Ruiz et Pav.) cv. Nash court in vitro were examined. Sucrose was the only medium component te sted that affected root emergence. Increasing the sucrose concentratio n in the medium from 10 to 30 g1(-1) stimulated adventitious root emer gence from rhizome buds that had been proliferated on a medium contain ing the gibberellin biosynthesis inhibitor paclobutrazol (5 mu M). Suc cessful clonal propagation of L. rosea cv. Nashcourt can now be achiev ed by a rhizome bud proliferation stage(s) in the presence of paclobut razol followed by an adventitious root emergence stage in the absence of paclobutrazol.