XCMI AS A UNIVERSAL RESTRICTION ENZYME FOR SINGLE-STRANDED-DNA

Single-stranded DNA can be cleaved into defined fragments at any prede termined site by interaction with a specially designed oligodeoxyribon ucleotide (oligo) adaptor and the class-IIN restriction endonuclease, XcmI. The oligo adaptor has the structure [GRAPHICS] Upon hybridizatio n to the target DNA through the central 9-nucleotide region and with t he addition of XcmI, the template DNA is specifically cleaved to near completion. Hairpin structures on the template close to the hybridizat ion site reduce the efficacy of cleavage.