EFFECT OF PHYSICOCHEMICAL PARAMETERS ON TURBIDIMETRIC ASSAY OF SCYLLIN-FETUIN INTERACTION

In order to optimise the interaction of scyllin, a low molecular weigh t lectin from Scylla serrata crab hemolymph, with fetuin the effect of various physicochemical parameters on the reaction has been studied t urbidimetrically. The formation of scyllin-fetuin complex is dependent on time, temperature, pH and ionic strength of the medium. The tubidi ty is maximum at 20-degrees-C, in 30 min and pH optimum is 7.5-8. The association constant (K(a)) of scyllin-fetuin interaction is 11.66x10( 5)M-1 at 20-degrees-C and 6.17x10(5)M-1 at 28-degrees-C. Among the dif ferent inorganic salts tested, KI has pronounced effect in binding. Di sodium salt increases the turbidity whereas monosodium salt decreases. Urea and periodate are inhibitory to scyllin-fetuin binding. Various alcohols and sugar alditols reduce the turbidity in scyllin-fetuin int eraction.