Dm. Aboulafia et al., HUMAN T-CELL LEUKEMIA-VIRUS (HTLV-I II) SERODIAGNOSTIC TESTING - DISPARATE RESULTS AMONG A COHORT OF INTRAVENOUS-DRUG-USERS/, AIDS research and human retroviruses, 9(10), 1993, pp. 1043-1050
Three hundred and forty-six sera collected over-a 2-year period from 1
54 San Francisco IV drug users were subjected to HTLV-I/II RIPA, Weste
rn blot (WB), Du Pont ELISA, and p24 radioimmunoassay (RIA). Tests wer
e performed at separate sites and code not broken until study end. RIP
A-positive and -negative controls consisted of Japanese adult T cell l
eukemia patients, healthy blood donors, and non-IV drug-using HIV-posi
tive men. RIPA identified HTLV-I/II-positive sera not identified by th
e other tests. Positive RIPAs were noted in 43% of negative ELISAs (n
= 279), 34% of negative WBs (n = 243), and 40% of negative RIAs (n = 2
70). Seventy-two sera were negative by all 3 assays, but were RIPA pos
itive. All sera positive by RIA (n = 76) and WB (n = 67), and 66 of 67
by ELISA, were positive by RIPA. Thirty-five of 36 indeterminate WBs
were RIPA positive. Seven samples indeterminate by RIPA were negative
by WB and RIA; one of seven was positive by ELISA. In all instances, s
amples negative by RIPA (n = 154) were ELISA, p24 RIA, and WB negative
or indeterminate. We conclude that when studying HTLV-I/II-endemic co
horts, screening ELISA or RIA followed by confirmatory WB or RIPA only
of seropositive samples may result in a substantial number of undetec
ted cases. Additional studies focusing on performance characteristics
of serodiagnostic tests are needed to ensure accurate inferences are m
ade in assessing HTLV-I/II prevalence rates among high-risk groups. Th
e RIPA may be a uniquely sensitive assay to detect HTLV-I/II gene-enco
ded products.