Abnormalities of the p53 gene and protein were examined in 81 primary
breast carcinoma samples. Using a polymerase chain reaction-single-str
and conformational polymorphism (PCR-SSCP) analysis, mutations in p53
exons 5-8 were identified in 13 of 81 tumours (16 per cent) and confir
med by DNA sequencing. Positive staining for p53 protein was detected
in ten of 77 (13 per cent) of these tumours using polyclonal CMI antib
ody on formalin-fixed tissue. Mutations detected by PCR-SSCP analysis
were more common in grade III tumours (P = 0.015), but no correlation
was found with tumour size, node status or level of epidermal growth f
actor receptor expression. A p53 mutation was associated with positive
antibody staining in only two patients. Positive immunohistochemical
staining using a p53 antibody may detect p53 protein expression, but t
his may not correlate directly with an underlying mutation in the hot
spot region examined.