MICRONUCLEUS INDUCTION IN HUMAN B-LYMPHOCYTE AND T-LYMPHOCYTE SEPARATED BY AN IMMUNOMAGNETIC TECHNIQUE

Micronuclei were induced in human lymphocytes by mitomycin C or gamma radiation, After the incubation the cells were separated in B- and T-c ells by an immunomagnetic method. i.e., Dynabeads. The cultures were s timulated by PHA. The effect of different incubation times (60-110 h) as well as different doses of mitomycin C (0-100 nmol/l) and gamma rad iation (0-2 Gy) at an incubation time of 72 h were studied. There were significantly higher frequencies of micronuclei in B- than in T-cells , when micronuclei were induced by mitomycin C. This was not found wit h gamma radiation, probably because of a cytotoxic effect of this agen t on the B-cells.