PROPERTIES AND CHARACTERIZATIONS OF STATI ONARY AND MOBILE PHASES USED IN REVERSED-PHASE LIQUID-CHROMATOGRAPHY

Nowadays, reversed phase liquid chromatography is the most employed ch romatographic technique. In comparison with gas chromatography, it off ers a greater power of separation because in addition there are solute -mobile phase interactions. As we can use phases of different natures (stationary as well as mobile phases), there are a lot of systems whic h can modi the solute-mobile phase, solute-stationary phase, as well a s mobile-stationary phases interactions and lead to a chromatographic analysis. In this article, we reviewed the characteristics and propert ies of these two kinds of phases. In the first part the main stationar y phases used in RPLC are listed with their synthesis and their charac terizations. The second part deals with the solvents of the mobile pha se. The different classifications which can be used to choose these so lvents will be discussed.