We have used interfacial force microscopy (IFM) to monitor the mechani
cal deformation of single nanometer-size grains in Au thin films. Our
results show that protruding grains, which represent early-stage delam
ination, display multiple deformation mechanisms including grain bound
ary sliding and intragranular plasticity. The unprecedented load-displ
acement control capability of the IFM provides data that are used for
the first time to quantitatively distinguish and evaluate individual d
eformation processes.