EXTRACELLULAR AND INTRACELLULAR LIPASES FROM A THERMOPHILIC RHIZOPUS-ORYZAE AND FACTORS AFFECTING THEIR PRODUCTION

A thermophilic Rhizopus oryzae was isolated, and parameters affecting its production of extra- and intra-cellular lipases were investigated. All carbon sources tested with the exception of sucrose generally inh ibited the production of extracellular lipase, but enhanced the produc tion of intracellular lipase. Peptone was the best substrate for extra cellular enzyme production, but for intracellular lipase production ot her substrates such as tryptone, tryptic soy digest, polypeptone, and corn steep liquor gave comparable results. Among lipid substrates, gly cerol was the only stimulator of extracellular enzyme production, wher eas olive oil, triolein, and oleic acid had very positive effects on i ntracellular enzyme production. Shaking enhanced the production of bot h types of enzymes; the temperature optima were 45 and 37-degrees-C fo r extra- and intra-cellular lipases, respectively. A pH of 5.0 was opt imal for production of both enzymes.