OPTIMIZATION OF BETA-GALACTOSIDASE EXTRACTION FROM KLUYVEROMYCES-MARXIANUS

Authors
STREDANSKY M TOMASKA M STURDIK E KREMNICKY L
Citation
M. Stredansky et al., OPTIMIZATION OF BETA-GALACTOSIDASE EXTRACTION FROM KLUYVEROMYCES-MARXIANUS, Enzyme and microbial technology, 15(12), 1993, pp. 1063-1065
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Enzyme and microbial technology → ACNP
ISSN journal
01410229
Volume
15
Issue
12
Year of publication
1993
Pages
1063 - 1065
Database
ISI
SICI code
0141-0229(1993)15:12<1063:OOBEFK>2.0.ZU;2-N
Abstract
The influence of several parameters, such as temperature, pH, and conc entration of buffer and solvent, on the release of beta-galactosidase from Kluyveromyces marxianus cells was studied. In optimal conditions (37-degrees-C, pH 9.5-10.5) greater than 90% of the intracellular beta -galactosidase activity was released into 0.1-0.5 M phosphate buffer a fter 1.5-2.0 h treatment with 1% chloroform. The described method is s imple, effective, relatively fast, and selective.