REQUIREMENT OF TYROSINE PHOSPHORYLATION FOR RAPID ACTIVATION OF A DNA-BINDING FACTOR BY IL-4

Citation
H. Kotanides et Nc. Reich, REQUIREMENT OF TYROSINE PHOSPHORYLATION FOR RAPID ACTIVATION OF A DNA-BINDING FACTOR BY IL-4, Science, 262(5137), 1993, pp. 1265-1267
Citations number
40
Categorie Soggetti
Multidisciplinary Sciences
Journal title
ISSN journal
00368075
Volume
262
Issue
5137
Year of publication
1993
Pages
1265 - 1267
Database
ISI
SICI code
0036-8075(1993)262:5137<1265:ROTPFR>2.0.ZU;2-4
Abstract
Interleukin-4 (IL-4) is an immunoregulatory cytokine produced by activ ated T lymphocytes to promote the growth and differentiation of cells that participate in immune defense. This study demonstrates the rapid activation of a specific DNA binding factor by IL-4. The IL-4 nuclear- activated factor (IL-4 NAF) appeared within minutes of IL-4 stimulatio n and recognized a specific DNA sequence found in the promoters of IL- 4-responsive genes. Activation of this putative transcription factor r equired tyrosine phosphorylation, and antibodies specific for phosphot yrosine recognize the IL-4 NAF-DNA complex. Thus, IL-4 appears to tran sduce a signal to the nucleus through tyrosine phosphorylation of a la tent DNA binding factor.