Color-flow and duplex ultrasonography were used to determine the optim
al method for documenting venous valvular reflux. Popliteal veins were
examined in 10 normal limbs and 11 limbs with clinical evidence of ch
ronic venous insufficiency (CVI). Peak reflux velocity (spectral) and
duration of reflux (spectral and color) were measured with the patient
in supine and standing positions, with manual and pneumatic compressi
on applied sequentially to thigh and calf. Manual and pneumatic compre
ssion produced equivalent reflux velocity and duration. In normal limb
s peak reflux velocity was always less than 22 cm/sec, with a mean rev
erse flow duration of 0.3 sec +/- 0.03 (SEM). In limbs with CVI, reflu
x velocity varied widely among protocols. Reflux duration and velocity
were greater in the supine position than in the standing position for
both normal limbs and limbs with CVI (p < 0.04). Duration was signifi
cantly increased for thigh versus calf compression in normal limbs (p
< 0.001) but decreased in limbs with CVI (p < 0.003). Methods that use
d thigh compression or supine position were less capable of discrimina
ting normal limbs from limbs with CVI. Standing calf compression provi
ded the greatest rates of sensitivity (91%), specificity (100%), and a
ccuracy (95%). Compared with spectral Doppler scanning, color-flow ult
rasonography produced a consistently shorter reflux duration (p < 0.00
1). In limbs with CVI with a mean spectral duration of 2.5 sec +/- 0.2
(SEM), mean color Doppler duration was 0.7 sec shorter. Our results d
emonstrate that popliteal vein incompetence is identified optimally by
reflux duration after standing calf compression; adequate manual comp
ression is sufficient to identify reflux; color-flow Doppler ultrasono
graphy may underestimate reflux duration.