EVIDENCE THAT CORTICOTROPIN-RELEASING FACTOR WITHIN THE EXTENDED AMYGDALA MEDIATES THE ACTIVATION OF TRYPTOPHAN-HYDROXYLASE PRODUCED BY SOUND STRESS IN THE RAT
Mc. Boadlebiber et al., EVIDENCE THAT CORTICOTROPIN-RELEASING FACTOR WITHIN THE EXTENDED AMYGDALA MEDIATES THE ACTIVATION OF TRYPTOPHAN-HYDROXYLASE PRODUCED BY SOUND STRESS IN THE RAT, Brain research, 628(1-2), 1993, pp. 105-114
Non-endocrine corticotropin-releasing factor (CRF) is believed to be i
nvolved in mediating stress behaviors in rats. The present study inves
tigated the role of CRF in mediating the activation of tryptophan hydr
oxylase, the rate-limiting enzyme in serotonin synthesis, produced in
response to sound stress. Bilateral injections of 0.5-3.0 mug of CRF d
irected towards the central nucleus of the amygdala increased tryptoph
an hydroxylase activity measured ex vivo when compared to vehicle-inje
cted controls. This increase in enzyme activity, like that due to soun
d stress. was reversed in vitro by alkaline phosphatase. Intra-amygdal
a CRF (0.5 mug) also enhanced the in vivo accumulation of 5-hydroxytry
ptophan (5-HTP) following the administration of m-hydroxylbenzylamine
(NSD-1015, 200 mg/kg). The activation of tryptophan hydroxylase. produ
ced hy intra-amygdala CRF, was blocked by the CRF receptor antagonist
alpha-helical CRF9-41 (10 mug). Additionally, the 5-HT1A agonist, gepi
rone, given either systemically (10 mg/kg) or intracerebrally into the
region of the dorsal raphe (14 mug), blocked the tryptophan hydroxyla
se response to CRF. CRF did not increase tissue levels of 5-hydroxyind
ole acetic acid (5-HIAA) or the ratio of 5-HIAA to serotonin (5-HT) wi
thin the striatum of the same animals in which tryptophan hydroxylase
activity was quantified, an effect produced by sound stress. Thus, whi
le intra-amygdala CRF failed to mimic the sound stress response in its
entirety, these data suggest that CRF is involved in mediating the ac
tivation of tryptophan hydroxylase produced by sound stress within the
midbrain serotonin neurons.