ANTIMUTAGENIC EFFECT OF RESVERATROL AGAINST TRP-P-1

The methanol extract of Yucca schidigera (YE) showed a suppressive eff ect on umu gene expression of the SOS response induced by 3-amino-1,4- dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) in Salmonella typhimurium TA 1535/pSK1002. The suppressive effect of YE was also observed for 2-ami noanthracene and activated Trp-P-1, without a significant effect on ba cterial growth. The extract exhibited a weak suppressive effect on SOS -induction by N-methyl-N'-nitro-N-nitrosoguanidine, but not by furylfu ramide or 4-nitroquinoline-1-oxide. The antimutagenic activity of YE a gainst Trp-P-1 was demonstrated by Ames assay using Salmonella typhimu rium TA98. Isolation and purification of the active component of YE wa s carried out using SiO2 column chromatography, and 275 mg of antimuta genic compound was isolated from 2.5 kg of dried chips of yucca roots and branches. The compound was identified as 3,4',5-trihydroxystilbene (THS). The SOS suppression and antimutagenicity of THS against Trp-P- 1 was determined by umu test and Ames test.