EFFECT OF AN ARYL CHLOROETHYL UREA ON TUBULIN AND VIMENTIN SYNTHESES IN A HUMAN BREAST-CANCER CELL-LINE

Citation
P. Poyet et al., EFFECT OF AN ARYL CHLOROETHYL UREA ON TUBULIN AND VIMENTIN SYNTHESES IN A HUMAN BREAST-CANCER CELL-LINE, Anticancer research, 13(5A), 1993, pp. 1447-1452
Citations number
35
Categorie Soggetti
Oncology
Journal title
ISSN journal
02507005
Volume
13
Issue
5A
Year of publication
1993
Pages
1447 - 1452
Database
ISI
SICI code
0250-7005(1993)13:5A<1447:EOAACU>2.0.ZU;2-I
Abstract
A new class of antineoplastic agents, 1 - aryl - 3 - (2 - chloroethyl) ureas (CEUs), was recently developed in our laboratory. To optimize t he pharmacological and the biological properties of this new class of compounds and to determine its mechanism of action, at the cellular le vel, we studied the effect of 4 - tert - butyl - [3 - (2 - chloroethyl ) ureido] benzene (tBCEU) on MDA - MB - 231, a human breast cancer hor mone - independent cell line. The effect of tBCEU on protein synthesis and on the accumulation of specific mRNAs was evaluated. The results indicate that tBCEU increases the synthesis of at least two proteins p resent in the cytoskeleton: tubulin and vimentin. The effect of tBCEU on their transcripts indicates that tBCEU decreases the accumulation o f tubulin and vimentin mRNA. These results suggest that the antineopla stic activity of tBCEU is in part related to an alteration in the synt hesis pathway of tubulin and vimentin.