VARIATIONS IN TEMPERATURE AND OXYGEN-CONTENT DO NOT ALTER LEVELS OF THIOBARBITURATE REACTIVE MATERIAL IN HUMAN BREAST-TUMOR CELLS (ZR-75-1)INCUBATED WITH GAMMA-LINOLENIC ACID

Authors
CANTRILL RC ELLS GW CHISHOLM KA HUGHES BJ HORROBIN DF
Citation
Rc. Cantrill et al., VARIATIONS IN TEMPERATURE AND OXYGEN-CONTENT DO NOT ALTER LEVELS OF THIOBARBITURATE REACTIVE MATERIAL IN HUMAN BREAST-TUMOR CELLS (ZR-75-1)INCUBATED WITH GAMMA-LINOLENIC ACID, Anticancer research, 13(5A), 1993, pp. 1461-1464
Citations number
18
Categorie Soggetti
Oncology
Journal title
Anticancer research → ACNP
ISSN journal
02507005
Volume
13
Issue
5A
Year of publication
1993
Pages
1461 - 1464
Database
ISI
SICI code
0250-7005(1993)13:5A<1461:VITAOD>2.0.ZU;2-2
Abstract
The mechanism by which tumour cells may be killed in vitro by exogenou s polyunsaturated fatty acids may involve lipid peroxidation. Gamma-li nolenic acid caused a dose and time-dependent reduction in ZR-75-1 cel l growth. However, altering either the incubator temperature (35, 37 a nd 39-degrees-C) or the oxygen content (16, 21 and 26%) had little eff ect on either the growth of cells in the presence of gamma-linolenic a cid or on thiobarbiturate reactive material levels over a 7 day period . Thus, small changes in cell culture conditions do not affect 18:3n-6 cytotoxicity or markers of lipid peroxidation.