KINETIC-ANALYSIS OF GLUCOSE-OXIDASE MONOM OLECULAR FILMS

Monomolecular films of glucose oxidase (GOD, EC 1.1.3.4) from Penicill ium vitale obtained on the basis of polymeric matrices were studied us ing the Clark-type oxygen electrode. The kinetic constants of the immo bilized and dissolved forms of GOD were compared. The kinetic activity of the enzyme in monomolecular films was studied depending on the con ditions of application, measurement and storage duration. The data obt ained indicate that GOD in monomolecular films formed on the positive- charge surface (polyethylenimine) has good kinetic characteristics. Th e sensitivity of the glucose electrodes varied in the range 1-10 mM of glucose, the time of analytical response was 20 sec. The immobilisati on of the enzyme led to decreased Michaelis constants (K(M)). The gluc ose electrodes retain their activity for 2 months at room temperature.