TREATMENT OF KRAFT BLEACHING EFFLUENTS BY LIGNIN-DEGRADING FUNGI .2. DETECTION OF NUCLEIC-ACID CONSTITUENTS IN THE EFFLUENT TREATED WITH THE LIGNIN-DEGRADING FUNGUS IZU-154

When a softwood kraft pulp bleaching effluent from the first alkaline extraction stage was treated with the fungus IZU-154, some nucleic aci d constituents, that could not be regarded as being derived from the e ffluent, were detected. These nucleic acid constituents were estimated to be two nucleobases (uracil and adenine) and three nucleosides (uri dine, guanosine and adenosine) with high-performance liquid chromatogr aphy. These nucleic acid constituents in E1 effluent treated with IZU- 154 were thought to be derived from yeast extract contained in a precu ltivation medium. Great decolorization of the E1 effluent was observed when nucleic acid constituents were abundantly detected in the treate d E1 effluent. In contrast to the results obtained with the fungus IZU -154, the nucleic acid constituents were hardly detected in the E1 eff luents treated with Coriolus versicolor (L. ex Fr.) Quel. and Phaneroc haete chrysosporium Burds. The phenomenon that the decolorization of E 1 effluent was well accompanied with the incorporation of the nucleic acid constituents into the fungus and the liberation of them to the tr eatment medium, was one of the characteristics of IZU-154.