SYNTHESIS OF POLYETHYLENE-GLYCOL OLIGONUC LEOTIDE CONJUGATES

A simple and fast method for the synthesis of 3'-, 5'-, and 3',5'-PEG- oligonucleotide conjugated molecules has been developed. Synthesis was carried out by a solid-phase procedure using automatic DNA synthesize r, or by the liquid-phase method. The H-phosphonate, phosphoramidite o r phosphotriester chemistry were used for the oligonucleotide chain el ongation. The regulated oligonucleotide conjugates obtained were used to study the efficiency of their penetration, stability and antisense effect in cell cultures and cell-free lysates.