A METHODOLOGICAL STUDY ON THE PREPARATION OF SUBCLASS-SPECIFIC ANTISERA TO BUFFALO IGG

Methods were tested for the preparation of monospecific antisera again st buffalo IgG1 and IgG2. The heterologous antisera raised in guinea p igs and rabbits showed extensive cross-reaction with the other subclas s. The cross-reacting antibodies were removed either by using immunoad sorbent or by addition of soluble protein of reciprocal subclass. The pre- and post- absorption antisera were tested for relative potency, s pecificity and degree of cross-reactivity by immunodiffusion, immunoel ectrophoresis and reverse radial immunodiffusion tests. The monospecif ic antisera obtained after addition of soluble proteins were better th an those obtained after adsorption with immunoadsorbent.