Kv. Kesari et J. Geliebter, REJUVENATED EXPRESSION OF H-2K(B) IN RMA-S CELLS DOES NOT AFFECT ALLOREACTIVE T-CELL-MEDIATED AND NATURAL-KILLER CELL-MEDIATED LYSIS, Immunology letters, 38(1), 1993, pp. 77-83
Reduced sensitivity to alloreactive cytotoxic lymphocytes (CTLs) and i
ncreased susceptibility to natural killer (NK) cells in vivo and in vi
tro are two phenotypic characteristics that have been attributed to th
e reduced major histocompatibility complex (MHC) ligand density on the
surface of an antigen-presentation-defective cell line, RMA-S. As RMA
-S exhibits both defective processing of antigenic peptides and very l
ow class I expression, it is uncertain which is responsible for the ab
ove characteristics. In this report, we show that the phenotype cannot
be reversed by increasing the number of K(b) + beta2-M complexes expr
essed on the cell surface. These findings emphasize the importance of
association of MHC class I molecules with endogenously processed pepti
des for biological interaction with alloreactive CTLs and NK cells.