INTERLEUKIN-1 AND TUMOR-NECROSIS-FACTOR ACTIVATE THE MITOGEN-ACTIVATED PROTEIN (MAP) KINASE KINASE IN CULTURED-CELLS

Interleukin 1 (IL1) activated mitogen-activated protein (MAP) kinase k inase in human gingival and foreskin fibroblasts and KB cells. Maximal activity was found in cytosolic extracts made after stimulating cells for 15 min. On anion-exchange chromatography two differently charged forms of MAP kinase kinase were identified, both phosphorylated a kina se-defective mutant MAP kinase, and activated recombinant wild type MA P kinase to phosphorylate MBP. Both were inhibited by an antiserum to recombinant MAP kinase kinase and the less acidic form was identified on Western blotting as an antigen of approximately 43 kDa. Indistingui shable forms were very much more strongly induced by phorbol myristate acetate (PMA). TNF had a similar effect to that of IL1.