MUTAGENIC SPECIFICITY OF THE (-DIOL EPOXIDE OF DIBENZ[A,J]ANTHRACENE IN THE SUPF GENE OF AN ESCHERICHIA-COLI PLASMID()ANTI)

This study was designed to examine the mutagenic specificity of (+)ant i-dibenz[a,j]anthracene 3,4-diol-1,2-epoxide ((+)anti-DB[a,j]A-DE) in SOS-induced repair-proficient Escherichia coli ES87 (DELTApro-lac, str A)/F'(pro+, lacl(Q), lacl(am26), lacZDELTAM15). The plasmid pUB3, whic h contains the mutation target gene, supF, was modified with (+)anti-D B[a,j]A-DE in vitro (two to five adducts/plasmid) and then transformed into bacteria by electroporation. The spontaneous mutation frequency for unmodified pUB3 in uninduced cells was about 2 X 10(-6) and for SO S-induced cells, about 8 x 10(-6) . The spontaneous supF- mutations we re primarily insertions, deletions, and frameshifts. The mutation freq uency for (+) anti-DB[a,j]A-DE-modified pUB3 was about 8 x 10(-6) and about 32 x 10(-6) for uninduced cells and SOS-induced cells, respectiv ely. (+)anti-DB[a,j]A-DE induced primarily point mutations in supF in SOS-induced cells. GC-AT transitions were the major mutations observed in SOS-induced cells (37%). GC-->TA (21%) and GC-->CG (8.6%) transver sion mutations were also observed, whereas mutations at AT base pairs were rare (1.9%). Furthermore, a large number of tandem GC/GC-AT/AT tr ansition mutations were also observed (about 15% of all mutations in S OS-induced cells). Taken together, single and tandem GC-->AT mutations accounted for slightly over half (about 51%) of the mutations observe d in SOS-induced cells. These results demonstrated that (+)anti-DB[a,j ]A-DE was mutagenic in repair-proficient E. coli, however, unlike othe r polycyclic aromatic hydrocarbons that induce primarily transversion mutations, (+)anti-DB[a,j]A-DE caused mostly GC-->AT transitions. (C) 1993 Wiley-Liss, Inc.