DETECTION OF CRYPTOSPORIDIUM-PARVUM USING A SPECIFIC POLYMERASE CHAIN-REACTION

Authors
WEBSTER KA POW JDE GILES M CATCHPOLE J WOODWARD MJ
Citation
Ka. Webster et al., DETECTION OF CRYPTOSPORIDIUM-PARVUM USING A SPECIFIC POLYMERASE CHAIN-REACTION, Veterinary parasitology, 50(1-2), 1993, pp. 35-44
Citations number
20
Categorie Soggetti
Parasitiology,"Veterinary Sciences
Journal title
Veterinary parasitology → ACNP
ISSN journal
03044017
Volume
50
Issue
1-2
Year of publication
1993
Pages
35 - 44
Database
ISI
SICI code
0304-4017(1993)50:1-2<35:DOCUAS>2.0.ZU;2-D
Abstract
The design and use of polymerase chain reaction primers and probes as reagents for the detection of Cryptosporidium parvum are described. Se nsitive and specific amplification of a 329 base pair product was demo nstrated by ethidium bromide staining and hybridisation of radiolabell ed probes. These reagents have the potential for application to diagno stic samples, environmental monitoring and epidemiological surveys.