EFFECTS OF PROSTAGLANDIN-E(1) ON THE PRODUCTION OF IGM AND IGG CLASS ANTI-DSDNA ANTIBODIES IN NZB W F(1) MICE/

Objective. To investigate the effects of prostaglandin E1 (PGE1) on Ig M and IgG class anti-dsDNA antibody production by young and aged femal e New Zealand black/white (NZB/W) F1 mouse spleen cells in vitro. Meth ods. Whole cells or B cells from NZB/W F1 mouse spleen cells were cult ured with lipopolysaccharide (LPS) in the absence or presence of grade d concentrations of PGE1 for 1 to 5 days. After cultures, the supernat ants were collected and assayed for released IgM and IgG class anti-ds DNA antibodies by enzyme-linked immunosorbent assay. Results. Young (3 -month-old) mouse spleen cells produced similar levels of IgM class an ti-dsDNA antibodies, while these cells produced considerably low level s of IgG class anti-dsDNA antibodies compared to aged (6-month-old) mo use spleen cells when stimulated with LPS. PGE1 suppressed the product ion of IgM class anti-dsDNA antibodies by about 50% at a concentration of 10(-6) M in both young and aged mouse spleen cell cultures. On the other hand, the production of IgG class anti-dsDNA antibodies was res istant to the inhibitory effects of PGE1. Conclusion. Our data suggest that PGE1 is effective in inhibiting the antibody synthesis by B cell s precommitted to IgM class anti-dsDNA antibody production, but the pr oduction of IgG class anti-dsDNA antibody by memory B cells present in young and aged mice is resistant to the inhibitory effects of PGE1.