T. Moroy et al., EXPRESSION OF A PIM-1 TRANSGENE ACCELERATES LYMPHOPROLIFERATION AND INHIBITS APOPTOSIS IN LPR LPR MICE, Proceedings of the National Academy of Sciences of the United Statesof America, 90(22), 1993, pp. 10734-10738
Transgenic mice expressing the Pim-1 kinase are predisposed to develop
T-cell lymphomas with a long latency period of about 7-9 months. Howe
ver, the exact functional basis of the oncogenic activity of Pim-1 rem
ains obscure. C57BL/6 mice homozygous for the lpr mutation develop a w
ell-described lymphoproliferative syndrome at about 26-30 weeks of age
. This syndrome is characterized mainly by the accumulation of abnorma
l T cells in lymph nodes because of the lack of Fas receptor-induced a
poptosis. We find that backcross of Emu-Pim-1 transgenics (mice with a
transgene that carries the mouse Pim-1 gene under the transcriptional
control of the immunoglobulin heavy chain gene enhancer Emu) into lpr
/lpr mice results in strong acceleration of lymphoproliferation and dr
amatic enlargement of lymph nodes. In addition, we show here that cult
ured lymph node cells from Emu-Pim-1 lpr/lpr mice are rescued from rap
id apoptosis that normally occurs in nontransgenic lpr cells in vitro.
We also present evidence that CD4+/CD8+ double-positive thymocytes fr
om lpr/lpr mice are sensitive to dexamethasone-induced apoptosis, alth
ough lpr/lpr mice lack the Fas receptor. In contrast, Emu-Pim-1 lpr/lp
r animals show considerable protection from dexamethasone-induced apop
tosis. These results show that Pim-1 can strongly accelerate lymphopro
liferation through inhibition of apoptosis and thereby provide first i
nsight into the functional basis for the oncogenic activity of Pim-1.