R. Karlsson et al., ANALYSIS OF ACTIVE ANTIBODY CONCENTRATION - SEPARATION OF AFFINITY AND CONCENTRATION PARAMETERS, Journal of immunological methods, 166(1), 1993, pp. 75-84
Antibody binding to surfaces with differing amounts of immobilised ant
igen was measured in a biosensor system using surface plasmon resonanc
e detection. Binding rates obtained during the initial binding phase o
n high density antigen surfaces were proportional to antibody concentr
ation and independent of antigen-antibody affinity. One antibody calib
ration curve covering the range from 0.5 to 160 nM (0.08-25 mu g/ml) a
ntibody was valid for IgG antibodies with different antigen specificit
ies. To illustrate the use of this methodology active antibody concent
rations were analysed in culture media and in rabbit serum.