CLONING OF TOXIC GENES WITH MINI-MU DERIVATIVE OF BACTERIOPHAGE MU

Authors
STUCHLIK S JANITOROVA V TURNA J
Citation
S. Stuchlik et al., CLONING OF TOXIC GENES WITH MINI-MU DERIVATIVE OF BACTERIOPHAGE MU, Acta virologica, 37(5), 1993, pp. 369-376
Citations number
25
Categorie Soggetti
Virology
Journal title
Acta virologica → ACNP
ISSN journal
0001723X
Volume
37
Issue
5
Year of publication
1993
Pages
369 - 376
Database
ISI
SICI code
0001-723X(1993)37:5<369:COTGWM>2.0.ZU;2-B
Abstract
We describe an in vivo cloning method using mini-Mu phage for genes, w hich cannot be cloned on multicopy vectors, mainly for their toxicity. We have successfully cloned succinate dehydrogenase (sdh) gene E. col i which was inactivated with defined insertion of fragment Km(r) by th is method. The most of obtained Km(r) clones of mini-Mu transductants have contained the sequence of whole sdh gene. The intact gene of sdh can be reconstructed by site-directed mutagenesis.