Pc. Swanson et Gd. Glick, CHEMICAL SEQUENCING OF DISULFIDE-CROSS LINKED OLIGODEOXYRIBONUCLEOTIDES, Bioorganic & medicinal chemistry letters, 3(10), 1993, pp. 2117-2118
Oligodeoxyribonucleotides containing a novel disulfide crosslink are s
hown to be amenable to chemical sequencing using standard sequencing r
eagents following reduction of the disulfide with DTT and selective al
kylation of the free thiols with N-ethylmaleimide. This strategy will
facilitate conformational and molecular recognition studies of DNA sta
bilized by crosslinking.