CHEMICAL SEQUENCING OF DISULFIDE-CROSS LINKED OLIGODEOXYRIBONUCLEOTIDES

Authors
SWANSON PC GLICK GD
Citation
Pc. Swanson et Gd. Glick, CHEMICAL SEQUENCING OF DISULFIDE-CROSS LINKED OLIGODEOXYRIBONUCLEOTIDES, Bioorganic & medicinal chemistry letters, 3(10), 1993, pp. 2117-2118
Citations number
17
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
3
Issue
10
Year of publication
1993
Pages
2117 - 2118
Database
ISI
SICI code
0960-894X(1993)3:10<2117:CSODLO>2.0.ZU;2-T
Abstract
Oligodeoxyribonucleotides containing a novel disulfide crosslink are s hown to be amenable to chemical sequencing using standard sequencing r eagents following reduction of the disulfide with DTT and selective al kylation of the free thiols with N-ethylmaleimide. This strategy will facilitate conformational and molecular recognition studies of DNA sta bilized by crosslinking.