INTERLEUKIN-10 INHIBITS VASCULAR-PERMEABILITY FACTOR RELEASE BY PERIPHERAL-BLOOD MONONUCLEAR-CELLS IN PATIENTS WITH LIPOID NEPHROSIS

The effect of recombinant human interleukin 10 (IL-10) on the in vitro secretion of vascular permeability factor (VPF) of peripheral blood m ononuclear cells (PBMC) was evaluated in patients with lipoid nephrosi s (LN). Significantly increased spontaneous and concanavalin A (ConA) stimulated secretion of VPF was detected in PBMC cultures of LN patien ts with the nephrotic syndrome as compared with those of normal contro ls. In the present study, IL-10 inhibited the ConA-stimulated VPF secr etion of PBMC in LN. A significant inhibitory effect of IL-10 was obse rved in cultures of PBMC from LN and IgA nephropathy patients as well as from normal persons. When both ConA and anti-IL-10 antibody were ad ded together to the PBMC, a further increase in ConA-stimulated secret ion of VPF occurred. These data suggest that IL-10 is a powerful inhib itor of the secretion of VPF in LN patients.