Zw. Zhang et al., ANTIBODY AGAINST SYNTHETIC RAT PTH PEPTIDE (1-34) BLOCKS PTH-MEDIATEDCAMP FORMATION AND PHOSPHATE-TRANSPORT IN OPOSSUM KIDNEY-CELLS, Nephron, 75(2), 1997, pp. 245-248
A novel antibody against synthetic rat parathyroid hormone (rPTH(1-34)
) was successfully produced in rabbits at a titer of 1:3,000, The abil
ity of this antibody to block PTH was studied utilizing the hormone-se
nsitive cAMP formation and Na+-dependent phosphate transport in the op
ossum kidney (OK) cells, rPTH peptide(1-34) stimulated cAMP formation
and inhibited Na+-dependent phosphate transport in OK cells in a dose-
dependent manner. Treatment of OK cells with the antisera significantl
y decreased the level of cAMP and attenuated the inhibition of Na+-dep
endent phosphate transport in response to rPTH(1-34) at a dilution of
1:1,000. Nonimmune rabbit sera at the same dilution did not influence
these hormone-sensitive effects. We conclude that antibody against syn
thetic PTH peptide can be used to study the biological activities of t
his hormone.